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Cell Signaling Technology Inc anti psyk
Anti Psyk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti psyk/product/Cell Signaling Technology Inc
Average 96 stars, based on 220 article reviews

anti psyk - by Bioz Stars, 2026-03

96/100 stars

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anti psyk (Cell Signaling Technology Inc)

Cell Signaling Technology Inc anti psyk
Anti Psyk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti psyk/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews

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96/100 stars

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rabbit anti psyk y352 65e4 id catalog (Cell Signaling Technology Inc)

Cell Signaling Technology Inc rabbit anti psyk y352 65e4 id catalog

(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk <t>Y352</t> (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.

Rabbit Anti Psyk Y352 65e4 Id Catalog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Anti Psyk Tyr525 526 Rabbit Monoclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti phospho syk psyk (Cell Signaling Technology Inc)

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Changes in the expression of activated receptor tyrosine kinases (RTKs) when AMAP1 was knocked down: The investigation of changes in activated RTK expression when AMAP1 was knocked down in HCC4006 ( A ) and A549 ( B ) cells using the activated RTKs assay. Each antibody was plotted in pairs, with the first six and last two representing the positive control. For A549 cells, owing to the low PD-L1 expression, stimulation was performed with TGF-β 1 ng/mL. Western blot analyses of pEGFR, <t>pSyk,</t> and pACK1 in HCC4006 ( C ) and A549 ( E ) cells. A comparison of pEGFR expression in HCC4006 ( G ) and A549 ( I ) cells when AMAP1 was knocked down, as observed through IF. Each result was semi-quantified using the ImageJ software ( D and F ) and the analysis application of BZ-X810 ( H and J ). The vertical axis represents the band ratio normalized to the brightness of the negative control (set to 1) ( D and F ). The vertical axis of the graph represents the cell count, and the brightness per cell was measured ( H and J ).

Anti Phospho Syk Psyk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psyk (Cell Signaling Technology Inc)

Cell Signaling Technology Inc psyk

( A ) Skin biopsies from patients with SSc ( n = 8) and individuals acting as healthy controls ( n = 4) were immunolabeled <t>with</t> <t>antibodies</t> against <t>pSyk</t> or ASMA, and immunofluorescence was visualized by Nikon A1R laser scanning confocal microscope. The percentage of immuno-positive cells (mean percentages from 4 randomly selected regions) was quantified. Mann-Whitney U test. P values are shown. Original magnification, ×40. ( B ) Confluent SSc skin fibroblasts ( n = 8, top; n = 3, bottom) were incubated with GF9 for 24 hours, and mRNA levels were quantitated by real-time quantitative PCR. Results were normalized with GAPDH and are shown as the mean ± SD of triplicate determinations from individual patients. Paired t test. P values are shown. ( C ) SSc fibroblasts ( n = 6) were immunolabeled using antibodies against collagen I, ASMA, or pSyk. Scale bar: 100 μm. Representative images and relative fluorescence intensities (mean ± SEM from 3 randomly selected regions) are shown.

Psyk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psyk/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews

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Image Search Results

(A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk Y352 (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: (A-C) Representative confocal micrographs showing a single z slice (∼280 nm) of IFN-γ-primed Csk AS BMDMs stained for total Lyn (yellow) and pSyk Y352 (cyan) after 3 min treatments with (A) medium only (untreated), (B) 3-IB-PP1, or (C) depleted zy-mosan (zym dep ). Fluorescence in the DAPI channel shows an internalized zym dep (magenta). Scale bars: 5 μm (D) Interaction area for pSyk Y352 clusters formed spontaneously during 3-IB-PP1 treatment and semi-contiguous interaction area per phagocytosed zym dep particle. Points are single-punctum values combined from 5 cells, shown with standard error of the mean (SEM). Significance (Sig.) in all panels assessed via nonparametric t test and Kolmogorov-Smirnov test: *** P = 0.0001. Inset: view of the lower range of 3-IB-PP1-induced pSyk clustering, showing the preponderance of nanoclusters within the distribution.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies (rabbit anti-pSyk Y352 65E4 ID/catalog # (#)2717, rabbit anti-pPLCγ Y1217 #3871, rabbit anti-pSHIP1 Y1020 #3941, rabbit anti-pSHP-1 Y564 D11G5 #8849, rabbit anti-pErk1/2 T202/Y204 D13.14.4e #4370, rabbit anti-pAkt S473 193H12 #4058, rabbit anti-pPI3K p85(Y458)/p55(Y199) #4228, mouse anti-β-Actin 8H10D10 #3700), Abcam (Cambridge, United Kingdom) (mouse anti-LynA+B Lyn01 ab1890), Santa Cruz (Dallas, TX, USA) (rabbit anti-LynA+B 44 #sc-15, goat anti-Hck M-28 #sc-1428), and LICOR (donkey anti-mouse IgG 800CW #925-32212, donkey anti-rabbit IgG 800CW #925-32213, donkey anti-mouse IgG 680LT #925-68022, donkey anti-goat IgG 680LT #926-32214 ) .

Techniques: Staining, Fluorescence

Densitometry quantification of protein species in immunoblotted lysates from IFN-γ-primed Csk AS BMDMs after 5 min treatment with 3-IB-PP1 or zym dep probed by immunoblot for (A) pSyk Y352 , (B) pPLCγ2 Y1217 , (C) pPI3K p85/p55 (D) immunoprecipitated Ras-GTP, (E) pErk1/2 Y202/T204 , and (F) pAkt S473 . Error: SEM; n=9-14 for 3-IB-PP1 and 11-15 for zym dep . Sig. assessed via non-parametric t test and Kolmogorov-Smirnov test: * P = 0.0216, *** P = 0.0007, ** P < 0.0001.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Densitometry quantification of protein species in immunoblotted lysates from IFN-γ-primed Csk AS BMDMs after 5 min treatment with 3-IB-PP1 or zym dep probed by immunoblot for (A) pSyk Y352 , (B) pPLCγ2 Y1217 , (C) pPI3K p85/p55 (D) immunoprecipitated Ras-GTP, (E) pErk1/2 Y202/T204 , and (F) pAkt S473 . Error: SEM; n=9-14 for 3-IB-PP1 and 11-15 for zym dep . Sig. assessed via non-parametric t test and Kolmogorov-Smirnov test: * P = 0.0216, *** P = 0.0007, ** P < 0.0001.

Techniques: Western Blot, Immunoprecipitation

(A-D) Immunoblots and quantifications of lysates from IFN-γ-primed Lyn +/+ and Lyn KO BMDMs probed for phosphorylated (A) Syk Y352 , (B) Erk1/2 T202/Y204 , (C) Akt S473 , and (D) SHIP1 Y1020 . β-Actin is a visual loading control, and quantifications were normalized to total protein content in the gel lane. Data are shown relative to the signal in Lyn +/+ . n=10-15 for Lyn +/+ and 6-7 for Lyn KO . Error bars: SEM. Sig. nonparametric, unpaired t with Kolmogorov-Smirnov test, *P=0.0152, **P≥0.007, ***P=0.0006 and ****P≤0.0001. (E) Representative images of PIP 3 (yellow) and nuclear staining (cyan) in IFN-γ -primed BMDMs. (F) Quantification of the geometric mean fluorescence intensity (gMFI) of PIP 3 staining in Lyn KO relative to Lyn +/+ BMDMs. Data points reflect the mean and SEM of 3-4 independent wells from 4 biological replicates. Sig. nonparametric, unpaired t test with Kolmogorov-Smirnov test, *** P<0.0006.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: (A-D) Immunoblots and quantifications of lysates from IFN-γ-primed Lyn +/+ and Lyn KO BMDMs probed for phosphorylated (A) Syk Y352 , (B) Erk1/2 T202/Y204 , (C) Akt S473 , and (D) SHIP1 Y1020 . β-Actin is a visual loading control, and quantifications were normalized to total protein content in the gel lane. Data are shown relative to the signal in Lyn +/+ . n=10-15 for Lyn +/+ and 6-7 for Lyn KO . Error bars: SEM. Sig. nonparametric, unpaired t with Kolmogorov-Smirnov test, *P=0.0152, **P≥0.007, ***P=0.0006 and ****P≤0.0001. (E) Representative images of PIP 3 (yellow) and nuclear staining (cyan) in IFN-γ -primed BMDMs. (F) Quantification of the geometric mean fluorescence intensity (gMFI) of PIP 3 staining in Lyn KO relative to Lyn +/+ BMDMs. Data points reflect the mean and SEM of 3-4 independent wells from 4 biological replicates. Sig. nonparametric, unpaired t test with Kolmogorov-Smirnov test, *** P<0.0006.

Techniques: Western Blot, Control, Staining, Fluorescence

Immunoblots and quantifications of (A-B) pSyk Y352 , (C-D) pErk1/2 T202/Y204 , (E-F) pAkt S473 , and (G-H) pSHIP1 Y1020 in IFN-γ-primed Csk AS (Lyn +/+ ) or Csk AS Lyn KO (Lyn KO ) BMDMs treated with (A, C, E, G) 3-IB-PP1 or (B, D, F, H) zym dep , corrected for total protein in each gel lane and shown relative to t=0 of Lyn +/+ ; n=10-11 for Lyn +/+ , 4-7 for Lyn KO . Error bars: SEM. Sig. via two-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0143, ** P = 0.0041, **** P<0.0001.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Immunoblots and quantifications of (A-B) pSyk Y352 , (C-D) pErk1/2 T202/Y204 , (E-F) pAkt S473 , and (G-H) pSHIP1 Y1020 in IFN-γ-primed Csk AS (Lyn +/+ ) or Csk AS Lyn KO (Lyn KO ) BMDMs treated with (A, C, E, G) 3-IB-PP1 or (B, D, F, H) zym dep , corrected for total protein in each gel lane and shown relative to t=0 of Lyn +/+ ; n=10-11 for Lyn +/+ , 4-7 for Lyn KO . Error bars: SEM. Sig. via two-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0143, ** P = 0.0041, **** P<0.0001.

Techniques: Western Blot

Immunoblots and quantification of (A) pSHIP1 Y1020 , (B) pAkt S473 , (C) pSyk Y352 , and (D) pErk T202/Y204 in CskAS (Hck +/+ Fgr +/+ ) and Csk AS Hck KO Fgr KO (Hck KO Fgr KO ) BMDMs, corrected for the total protein in each lane and shown relative to t=0 of Hck +/+ Fgr +/+ . Error bars: SEM, n=10-11 for Hck +/+ Fgr +/+ and n=4 for Hck KO Fgr KO . Sig. 2-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0499.

Journal: bioRxiv

Article Title: Steady-state phosphorylation of SHIP1 by Lyn restricts macrophage activation in the absence of a phagocytic synapse

doi: 10.1101/2025.04.16.648985

Figure Lengend Snippet: Immunoblots and quantification of (A) pSHIP1 Y1020 , (B) pAkt S473 , (C) pSyk Y352 , and (D) pErk T202/Y204 in CskAS (Hck +/+ Fgr +/+ ) and Csk AS Hck KO Fgr KO (Hck KO Fgr KO ) BMDMs, corrected for the total protein in each lane and shown relative to t=0 of Hck +/+ Fgr +/+ . Error bars: SEM, n=10-11 for Hck +/+ Fgr +/+ and n=4 for Hck KO Fgr KO . Sig. 2-way ANOVA with Sidak’s multiple comparisons test: * P = 0.0499.

Techniques: Western Blot

Journal: Cancer Management and Research

Article Title: ArfGAP with the SH3 Domain, Ankyrin Repeat and PH Domain 1 Inversely Regulates Programmed Death-Ligand 1 Through Negative Feedback of Phosphorylated Epithelial Growth Factor Receptor and Activation of Nuclear Factor-Kappa B in Non-Small Cell Lung Cancer

doi: 10.2147/CMAR.S493368

Figure Lengend Snippet: Changes in the expression of activated receptor tyrosine kinases (RTKs) when AMAP1 was knocked down: The investigation of changes in activated RTK expression when AMAP1 was knocked down in HCC4006 ( A ) and A549 ( B ) cells using the activated RTKs assay. Each antibody was plotted in pairs, with the first six and last two representing the positive control. For A549 cells, owing to the low PD-L1 expression, stimulation was performed with TGF-β 1 ng/mL. Western blot analyses of pEGFR, pSyk, and pACK1 in HCC4006 ( C ) and A549 ( E ) cells. A comparison of pEGFR expression in HCC4006 ( G ) and A549 ( I ) cells when AMAP1 was knocked down, as observed through IF. Each result was semi-quantified using the ImageJ software ( D and F ) and the analysis application of BZ-X810 ( H and J ). The vertical axis represents the band ratio normalized to the brightness of the negative control (set to 1) ( D and F ). The vertical axis of the graph represents the cell count, and the brightness per cell was measured ( H and J ).

Article Snippet: The following primary antibodies were used: Anti‐phospho-epidermal growth factor receptor (pEGFR) (Cell Signaling Technology; cat. no. 2236 1:1000), anti‐PD-L1 (Cell Signaling Technology; cat. no. 13684 1:1000), anti- phospho-ACK1 (pACK1) (Cell Signaling Technology; cat. no. 3138 1:500), and anti‐phospho-Syk (pSyk) (Cell Signaling Technology; cat. no. 2710 1:1000), anti‐DDEF1 (Santa cruz; cat. sc-374410 1:1000), anti‐NF-κB (Santa cruz; cat. sc-8008 1:1000) and anti‐β‐actin (Millipore Sigma; cat. no. A5441 1:2000).

Techniques: Expressing, Positive Control, Western Blot, Comparison, Software, Negative Control, Cell Counting

( A ) Skin biopsies from patients with SSc ( n = 8) and individuals acting as healthy controls ( n = 4) were immunolabeled with antibodies against pSyk or ASMA, and immunofluorescence was visualized by Nikon A1R laser scanning confocal microscope. The percentage of immuno-positive cells (mean percentages from 4 randomly selected regions) was quantified. Mann-Whitney U test. P values are shown. Original magnification, ×40. ( B ) Confluent SSc skin fibroblasts ( n = 8, top; n = 3, bottom) were incubated with GF9 for 24 hours, and mRNA levels were quantitated by real-time quantitative PCR. Results were normalized with GAPDH and are shown as the mean ± SD of triplicate determinations from individual patients. Paired t test. P values are shown. ( C ) SSc fibroblasts ( n = 6) were immunolabeled using antibodies against collagen I, ASMA, or pSyk. Scale bar: 100 μm. Representative images and relative fluorescence intensities (mean ± SEM from 3 randomly selected regions) are shown.

Journal: JCI Insight

Article Title: Inhibiting triggering receptor expressed on myeloid cells 1 signaling to ameliorate skin fibrosis

doi: 10.1172/jci.insight.176319

Figure Lengend Snippet: ( A ) Skin biopsies from patients with SSc ( n = 8) and individuals acting as healthy controls ( n = 4) were immunolabeled with antibodies against pSyk or ASMA, and immunofluorescence was visualized by Nikon A1R laser scanning confocal microscope. The percentage of immuno-positive cells (mean percentages from 4 randomly selected regions) was quantified. Mann-Whitney U test. P values are shown. Original magnification, ×40. ( B ) Confluent SSc skin fibroblasts ( n = 8, top; n = 3, bottom) were incubated with GF9 for 24 hours, and mRNA levels were quantitated by real-time quantitative PCR. Results were normalized with GAPDH and are shown as the mean ± SD of triplicate determinations from individual patients. Paired t test. P values are shown. ( C ) SSc fibroblasts ( n = 6) were immunolabeled using antibodies against collagen I, ASMA, or pSyk. Scale bar: 100 μm. Representative images and relative fluorescence intensities (mean ± SEM from 3 randomly selected regions) are shown.

Article Snippet: For immunofluorescence, paraffin-embedded skin sections were incubated with antibodies against ASMA (Sigma-Aldrich, 1:100, A5228), pSyk (CST 2710S, 1:100), pDAP12 (ab314891, 1:100), CD45 (14-0451-82, 1:100), CD3(sc-20047; 1:100), TREM-1 (Invitrogen PA5-47090, 1:100), anti-CD11b antibody (Abcam AB133357, 1:100), procollagen I (Sigma-Aldrich, MAB1912 1:200), or perilipin (Abcam; ab61682) followed by appropriate secondary antibodies.

Techniques: Immunolabeling, Immunofluorescence, Microscopy, MANN-WHITNEY, Incubation, Real-time Polymerase Chain Reaction, Fluorescence